A landmark review, now published in the Journal of Experimental & Clinical Cancer Research, offers a sweeping and authoritative synthesis on the use of liquid biopsy in gynecological oncology placing this emerging tool at the forefront of precision medicine for women. Led by Professor Antonio Giordano, President of the Sbarro Health Research Organization (SHRO) and molecular oncologist at Temple University and the University of Siena, the study lays out a rigorous translational roadmap to bridge cutting-edge molecular diagnostics with real-world clinical application.
The article, titled "Liquid biopsy in gynecological cancers: a translational framework from molecular insights to precision oncology and clinical practice[1]," presents a decisive shift from mere technical exploration to clinically actionable guidance.
It systematically addresses how liquid biopsy, through circulating tumor DNA (ctDNA), tumor-educated platelets, microRNAs, and extracellular vesicles, can revolutionize the diagnosis, monitoring, and therapeutic tailoring of endometrial, cervical, and ovarian cancers.
What distinguishes this review is its explicit focus on translational relevance. It does not limit itself to theoretical or laboratory insights. Rather, it rigorously maps fourteen critical fields of inquiry from assay design and biomarker validation to clinical endpoints and cost-effectiveness with a central aim: enabling reliable, standardized integration of liquid biopsy into routine oncology care.
The authors argue that liquid biopsy is not merely an innovation in biomarker detection, it is a methodological bridge that aligns molecular biology with patient-centered care. This is especially vital in gynecological cancers, where the heterogeneity of disease and anatomical constraints often hinder early diagnosis and longitudinal monitoring. By offering a noninvasive, repeatable, and dynamic window into tumor biology, liquid biopsy has the potential to transform the clinical management of cancers that impact over 1.3 million women in the United States alone.
The implications for women's health are particularly profound. For ovarian cancer, where early-stage detection remains notoriously difficult, liquid biopsy demonstrates up to 90% specificity and increasing sensitivity through combined microRNA and ctDNA panels. In endometrial cancer, personalized ctDNA analysis shows over 87% concordance with traditional tissue-based classification and can predict recurrence several months in advance of clinical imaging.
In cervical cancer, circulating HPV DNA and specific serum protein levels such as SCC-Ag and VCAM-1 correlate with prognosis and therapy response, reinforcing the value of plasma-based surveillance. Moreover, ctDNA profiling has proven effective in tracking chemotherapy resistance, especially in BRCA-mutated tumors treated with PARP inhibitors.
These findings underscore not only the scientific innovation, but the gendered urgency of the work: the application of liquid biopsy in gynecological malignancies is not an abstract advance, but a concrete opportunity to improve survival, reduce diagnostic invasiveness, and individualize treatment for millions of women.
Overcoming Barriers, Building Integration
Despite its promise, the authors emphasize that liquid biopsy remains underutilized in clinical gynecologic oncology. The review therefore presents detailed considerations to overcome the barriers that prevent translation from the need for FDA-approved multi-analyte assays to standardization of sample handling and AI-driven data interpretation.
The article calls for rigorous clinical trials that move beyond biomarker performance in advanced disease, toward validation in early-stage settings and population-level screening. Particular attention is paid to the integration of fragmentomics, methylomics, and machine learning in refining diagnostic precision.
Reference:
1] https://jeccr.biomedcentral.com/articles/10.1186/s13046-025-03371-1
(Newswise/TK)