EAST LANSING, Mich. — Michigan State University researchers have solved the mystery of a poorly understood sperm structure called the cytoplasmic droplet, or CD. The CD is an expanded cytoplasm — watery, gel-like cell contents enclosed by cell membrane — found close to the head, at the neck of the sperm, in all mammals, including humans. This new genetic model is the first of its kind.
Despite being first discovered more than 100 years ago, it’s been unclear how the CD forms and what its function is due to the lack of molecular and genetic tools to study it.
Scientists have often disregarded it, but it can’t be ignored anymore said Chen Chen, an associate professor in the Department of Animal Science and the Reproductive and Developmental Sciences Program in MSU’s College of Agriculture and Natural Resources.
“Our new study using mice shows that the CD is indeed an actively forming organelle purposefully designed to regulate sperm maturation and fertility,” Chen said. “It acts as a ‘storage room’ to pack up critical proteins needed for sperm function before its long journey to reach the egg. It’s like packing for a trip to Mars — the spaceship needs to load up everything needed for the journey before leaving Earth.”
Chen noted that when the SYPL1 gene is deleted in mice, this protein trafficking system collapses, and critical metabolic enzymes and essential proteins fail to be shipped to the CD, resulting in an empty CD devoid of vesicles — something he had never seen before. Consequently, without major CD contents, mutant sperm lose their motility and ability to cope with osmotic changes — they bend, leading to infertility.
“Fundamentally, this finding will change people’s misconception about this mysterious sperm structure. It will open new avenues for research on the CD as a biomarker for fertility in humans and animals and, possibly, on targeting this novel pathway for fertility control.”
The study is published in the journal Nature Communications, and the research was funded in part by the National Institutes of Health and MSU AgBioResearch. Chen’s team also includes collaborators from the University of Illinois, McGill University and China Agricultural University.
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