Bryan Johnson’s Latest Biohacking Experiment Turns His Urine Blue: What Is Methylene Blue and Why Is He Taking It?

Tech mogul and anti-aging enthusiast Bryan Johnson is once again making headlines—but this time, it’s not for his strict 2,250-calorie diet, $2 million longevity protocol, or plasma infusions from his teenage son. The 47-year-old entrepreneur recently revealed that his latest biohacking experiment involves methylene blue, a synthetic compound turning his urine bright blue.

While the color change sounds bizarre, Johnson claims it could be a sign of a deeper transformation—one aimed at enhancing cognitive function, mitochondrial health, and potentially extending human lifespan.

Methylene blue is a synthetic dye first developed in the 19th century. It originally found use in textile coloring, but quickly gained medical relevance. According to the U.S. Food and Drug Administration (FDA), methylene blue is approved for treating methemoglobinemia, a rare blood disorder that reduces the blood’s ability to carry oxygen. It's also used in cases of cyanide poisoning, malaria, and for some neurological conditions.

In recent years, however, the compound has attracted attention from biohackers and longevity researchers due to its proposed effects on mitochondrial function—the “powerhouse” of the cell.

Bryan Johnson’s experiment with methylene blue fits into his larger Blueprint project, a meticulously data-driven and science-focused protocol aimed at reversing his biological age. Johnson claims to have the heart of a 37-year-old, lungs of an 18-year-old, and skin comparable to that of a 10-year-old—all thanks to his extreme anti-aging regimen.

Johnson reportedly uses methylene blue for its nootropic (cognitive-enhancing) and neuroprotective potential, believing it might support better cellular energy metabolism, memory, and even slow the aging process.

While methylene blue has legitimate clinical uses, its off-label application as an anti-aging compound is still under investigation. Preliminary research suggests that in low doses, methylene blue may enhance mitochondrial respiration, reduce oxidative stress, and improve cognitive performance in animal studies and limited human trials.

However, experts caution that these effects are not yet conclusive, and long-term safety data is lacking. Dr. Ana Maria Cuervo, co-director of the Einstein Institute for Aging Research, stated in interviews that “we need more rigorous clinical trials before recommending it as a longevity intervention.”

Yes. Methylene blue is not without potential dangers:

• Serotonin Syndrome: When combined with SSRIs or other antidepressants, it can trigger a potentially fatal condition.

• G6PD Deficiency: Individuals with this genetic disorder may suffer severe side effects, including hemolytic anemia.

• Kidney Conditions & Pregnancy: It’s considered unsafe for people with renal disease or for pregnant women.

According to Harvard Health Publishing, methylene blue’s interaction profile is complex, and unsupervised use—especially from unregulated supplements—can be dangerous.

Methylene blue is just one tool in Johnson’s longevity toolkit. His Blueprint regimen includes:

• Over 100 supplements daily, targeting inflammation, hormone levels, and cellular repair.

• Calorie-restricted vegan diet, optimized for nutrient density.

• Intermittent Hypoxia-Hyperoxia Training (IHHT): Alternates between low and high oxygen levels to potentially boost mitochondrial resilience and cardiovascular health.

• Blood plasma infusions: Previously from his teenage son, now halted following scientific backlash.

Johnson’s highly publicized experiments are fueling a growing debate: How far should science go in the quest to defeat aging? His methods, while extreme and controversial, are sparking global curiosity—and scrutiny—around the future of human longevity.

While methylene blue’s dramatic side effect makes for viral headlines, the real question remains: Is it a glimpse into the future of anti-aging medicine—or simply another blue-tinted fad?

Whether you're intrigued or skeptical, Bryan Johnson’s biohacking journey highlights the cutting edge (and ethical gray areas) of longevity science. As with all experimental therapies, medical supervision, scientific rigor, and skepticism are critical.

(Rh/Eth/VK/MSM/SE)